FC Dallas will be facing off against Nashville SC at 7:30 p.m. in the opener of two back-to-back matchups.
Both teams were forced to withdraw from the recent MLS is Back tournament due to Covid-19 outbreaks among staff and players.
MLS is the first major team sports league to host a game in front of fans since the coronavirus pandemic forced leagues to suspend or delay their respective seasons in March.
The NBA, NHL and MLB have resumed or begun their seasons, but there have been no fans in attendance.
Cash is not accepted at concession stands in order to minimize physical interaction, the team said. No paper tickets are being distributed, as all tickets will be delivered and processed through the AXS ticketing app.
Texas currently has recorded at least 518,150 confirmed coronavirus cases -- third most among US states -- and more than 9,225 deaths, according to a tally by Johns Hopkins University.